rabbit anti ngal  (Proteintech)

Journal: mBio

Article Title: Inhibition and evasion of neutrophil microbicidal responses by Legionella longbeachae

doi: 10.1128/mbio.03274-24

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: After fixation and permeabilization, cells to be analyzed for granule fusion were labeled with goat anti- L. pneumophila antibody (1:1,000 dilution), chicken anti- L. longbeachae antibody (1:4,000 dilution), rabbit anti-lactoferrin antibody (1:100 dilution, Abcam #Ab109216), rabbit anti-NGAL antibody (1:100 dilution, ThermoFisher #PA5-79589), rabbit anti-CEACAM8/Cd66b antibody (1:200 dilution, ThermoFisher # PA5-104296), and mouse anti-elastase antibody (1:100 dilution, Abcam #Ab254178).

Techniques:

Journal: mBio

Article Title: Inhibition and evasion of neutrophil microbicidal responses by Legionella longbeachae

doi: 10.1128/mbio.03274-24

Figure Lengend Snippet: Fusion of specific granules to the L. longbeachae -containing phagosome. To determine the fusion of specific granules to bacterial phagosomes, neutrophils were labeled for lactoferrin ( A–C ) and NGAL ( D–F ). Neutrophils were solo-infected with WT or Δ T4 bacterial strains for 15 min or subjected to 15 min primary infections (1°) followed by super-infection (2°) for an additional 15 minutes. Representative confocal images of specific granule fusion to phagosomes during solo-infections ( A and D ) or super-infections ( B and E ). Bacteria were labeled with anti- Llo antibody (cyan) or anti- Lpn antibody (green). Zymosan A BioParticles Alexa Fluor 594 are shown in green. Specific granules were labeled with anti-lactoferrin or anti-NGAL antibodies (red). White arrows indicate positive co-localization of lactoferrin or NGAL with pathogen-containing phagosomes. ( C and F ) Data are shown as mean percent co-localization of lactoferrin or NGAL to Llo phagosomes (red) or Lpn phagosomes (blue) during infections. Zymosan was included as a positive control (green) ± SD, n = 3 (scatter plot dots). The data shown are representative of three independent biological repeats.

Article Snippet: After fixation and permeabilization, cells to be analyzed for granule fusion were labeled with goat anti- L. pneumophila antibody (1:1,000 dilution), chicken anti- L. longbeachae antibody (1:4,000 dilution), rabbit anti-lactoferrin antibody (1:100 dilution, Abcam #Ab109216), rabbit anti-NGAL antibody (1:100 dilution, ThermoFisher #PA5-79589), rabbit anti-CEACAM8/Cd66b antibody (1:200 dilution, ThermoFisher # PA5-104296), and mouse anti-elastase antibody (1:100 dilution, Abcam #Ab254178).

Techniques: Labeling, Infection, Bacteria, Positive Control

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: Silencing HE4 alleviates the renal fibrosis in lupus nephritis mice by regulating the C3/MMPs/prss axis

doi: 10.1007/s00210-023-02883-x

Figure Lengend Snippet: MRL/LPR mice were injected with the adenovirus containing the shRNA-NC or HE4 shRNA. The expression of β2-MG, NGAL, and Kim-1 in renal tissues was detected using the immunohistochemical assay ( n =3, ×400, * p <0.05 vs. control, # p <0.05 vs. model, @ p <0.05 vs. M+ NC)

Article Snippet: After blocking with 5% BSA, sections were incubated with rabbit anti-β2-MG antibody (1:200, 13511-1-AP, Proteintech, USA), rabbit anti-NGAL antibody (1:200, PB9609, BOSTER, USA), and rabbit anti-Kim-1 antibody (1:200, bs-21420R, Bioss, USA) overnight at 4°C.

Techniques: Injection, shRNA, Expressing, Immunohistochemical staining, Control